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課程名稱 |
次世代定序分析
Next Generation Sequencing Analysis |
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開課學期 |
112-2 |
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授課對象 |
生命科學院 生命科學院院學士學位 |
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授課教師 |
林友瑜 |
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課號 |
GenSys5024 |
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課程識別碼 |
B48EU0390 |
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班次 |
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學分 |
3.0 |
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全/半年 |
半年 |
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必/選修 |
選修 |
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上課時間 |
星期二2,3,4(9:10~12:10) |
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上課地點 |
生科4B |
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備註 |
本課程以英語授課。地點:生科院4B教室
總人數上限：30人
外系人數限制：15人 |
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課程簡介影片 |
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核心能力關聯 |
核心能力與課程規劃關聯圖 |
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課程大綱
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課程概述 |
During the past decade, large sequence data sets generated by next generation sequencing (NGS) has become the mainstream method to obtain and study genomic information. This course will cover the concept, methodology, algorithms, and current advances and tools for handling NGS generated data sets.
[Classroom changed to 4A] |
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課程目標 |
Upon completion of the course, students should gain a more comprehensive understanding of the overall process of next generation sequencing starting from sample preparation to data analysis. |
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課程要求 |
Homework assignments 30%
Midterm exam 35%
Final exam 35%
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預期每週課後學習時數 |
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Office Hours |
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指定閱讀 |
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參考書目 |
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評量方式
(僅供參考) |
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週次 |
日期 |
單元主題 |
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Week 1 |
2/20 |
Introduction |
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Week 2 |
2/27 |
Principles of next generation sequencing |
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Week 3 |
3/5 |
NGS archives, quality control and normalization |
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Week 4 |
3/12 |
Data assembly, alignment, and annotation |
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Week 5 |
3/19 |
Detection and comparison of mutational profiles |
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Week 6 |
3/26 |
Comparison of expression profiles |
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Week 7 |
4/2 |
Liquid biopsy |
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Week 8 |
4/9 |
Midterm exam |
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Week 9 |
4/16 |
Single cell sequencing |
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Week 10 |
4/23 |
Genome wide association studies |
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Week 11 |
4/30 |
Epigenetic modification analyses |
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Week 12 |
5/7 |
Splicing, viral integrations and genomic structural variations |
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Week 13 |
5/14 |
Incorporation and development of analytic pipelines |
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Week 14 |
5/21 |
Visit to Biotools (圖爾思 https://www.toolsbiotech.com/) |
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Week 15 |
5/28 |
Visit to IMB Bioinformatics Core, Academia Sinica (https://bc.imb.sinica.edu.tw/) |
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Week 16 |
6/4 |
Final exam/report |
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